Description
Aviva Systems Biology Tnmd ELISA Kit (Rat) (OKEH04398) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tnmd antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tnmd and incubated. The found in the sample or standards competes with the biotinylated Tnmd for limited binding sites on the immobilized anti-Rat Tnmd antibody. Excess unbound biotinylated Tnmd and sample or standard Tnmd is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tnmd captured in the well and inversely proportional to the amount of Tnmd which was contained in the sample or standard